Nexera GPC Gel Permeation Chromatography System

Nexera GPC Gel Permeation Chromatography System

Integrating Analytical Intelligence with Exceptional Instrument Performance

The Nexera GPC System provides highly reliable and highly extensible performance in a wide variety of applications. Hardware that provides rapid instrument stabilization and excellent reproducibility of analytical results, and software that includes analytical workflow automation and overlap injection functions and features a user-friendly analysis screen view contribute to improving productivity. In addition, Analytical Intelligence functionality based on state-of-the-art digital technology provides highly reliable analytical results by preventing potential problems and ensuring the instrument is always in a stable state, regardless of the skill, knowledge, or experience level of the analyst.

Features

 

Increased Throughput by Overlapped Injection

Measuring molecular weight distribution of polymer compound by size exclusion mode is one of the typical parts of HPLC and generally called gel permeation chromatography (GPC). Nowadays there is an increasing demand demand for high throughput analysis even in well-established GPC. Here we introduce a novel GPC analysis that affords both high throughput GPC results by overlapped injection using conventional size of columns and simultaneous determination of polymer additives.

 

GPC Analysis by Overlapped Injection and Simultaneous Determination of Polymer Additives

A refractive index detector (RID) that gives a response to a sample weight is commonly employed for GPC to calculate average molecular weight and polydispersity. On the other hand, A UV detector is used for determination of polymer additives such as antioxidants. Consequently, serially connected those two detectors were used for this study. In GPC, there is hardly any eluates prior to exclusion limit. So if the sample elution band from previous injection is managed to be overlapped within this no elution interval in present analysis, short analysis cycle time can be obtained and it provides increased throughput in sequential analyses. When two or more polymer additives are contained, correct determination of those small additives is difficult because complete separation of additives is almost impossible even using GPC columns of small exclusion limit due to small difference of molecular weight among those polymer additives. To address this difficulty, a photodiode array detector (PDA) that affords spectrometric information as well as chromatographic results was employed to give increased separation of polymer additives by peak deconvolution function within a single GPC analysis.

Fig. 1 and Table 1 show obtained chromatogram of polystyrene containing three antioxidants and employed analytical conditions respectively.

 
Chromatogram of Polystyrene and Three Additives (RID)

Fig. 1 Chromatogram of Polystyrene and Three Additives (RID)

 

Table 1 Analytical Conditions
Column
Shim-pack™ GPC 805+GPC 801
(300 mmL. × 8 mmI.D. for each)
Mobile phase THF
Flow rate 0.8 mL/min
Column temp. 40 °C
Injection vol. 10 μL
Detection RID / PDA (220-400 nm)
Sample 0.5 % Polystyrene containing three additives
Cycle time 31 min
Overlap time 15.5 min

Increased Throughput by Overlapped Injection

Based on the chromatogram in Fig. 1, 0 to 15.5 term was assigned to overlapped interval due to no elution during it. Fig. 2 shows the comparison of sequential chromatograms obtained with/without overlapped injection. Almost 50 % of decreased analysis cycle time provided increased throughput of sequential GPC analyses.

Comparison of Sequential GPC Analyses With (Lower) / Without (Upper) Overlapped Injection (RID)

Fig. 2 Comparison of Sequential GPC Analyses With (Lower) / Without (Upper) Overlapped Injection (RID)

 
 
 

Ensures Highly Reliable Analysis Regardless of Operator Skill Level

Protects Expensive GPC Columns:FlowPilot  ANALYTICAL INTELLIGENCE

FlowPilot

HPLC columns can be damaged by sudden pump starts and stops or extreme gradient changes. The Nexera automatically uses FlowPilot (Smart Flow Control) to increase the flow rate gradually to the set point. There is no need to create startup protocols for each analysis.

Mobile Phase Levels Measured in Real Time ANALYTICAL INTELLIGENCE

Reservoir tray weight sensors (optional) can be used to monitor the volume of mobile phase or autosampler rinse solution in up to twelve containers. The containers can also be checked remotely from a smart device. You will no longer need to worry about running out of mobile phase mid-analysis, because the device will notify you before starting the run if the volume remaining is too low.

Mobile phase level can be monitored from outside the laboratory

Auto-Diagnostics and Recovery ANALYTICAL INTELLIGENCE

In rare cases, air bubbles can form in the mobile phase and cause problems if inhaled into the pump. The Nexera has the ability to monitor baseline changes and pressure fluctuations to check for abnormalities. When it detects an unusual fluctuation, it can automatically pause the analysis, purge the flow path, and restart analysis once it has confirmed recovery to normal pressure.

Auto-Diagnostics and Recovery

 
 

Automated Workflow Analytical Intelligence

Automated Workflow

Functionality for assisting the entire series of analytical process steps, from instrument startup to shutdown, significantly increases work efficiency. FlowPilot mobile phase flowrate control functionality is linked to column oven temperature, so that columns are protected during automatic column equilibration. By automatically checking for baseline drift, instrument stability is maintained without the operator having to remain next to the instrument.

 

ANALYTICAL INTELLIGENCE Analytical Intelligence Logo

  • Automated support functions utilizing digital technology, such as M2M, IoT, and Artificial Intelligence (AI), that enable higher productivity and maximum reliability.
  • Allows a system to monitor and diagnose itself, handle any issues during data acquisition without user input, and automatically behave as if it were operated by an expert.
  • Supports the acquisition of high-quality, reproducible data regardless of an operator’s skill level for both routine and demanding applications.
 

Applications

Chromatogram of Polystyrene and Three Additives

Increased Throughput with Nexera™ GPC system: Overlapped Injection and Simultaneous Determination ofPolymer Additives

Measuring molecular weight distribution of polymer compound by size exclusion mode is one of the typical parts of HPLC and generally called gel permeation chromatography (GPC). Nowadays there is an increasing demand demand for high throughput analysis even in well-established GPC. Here we introduce a novel GPC analysis that affords both high throughput GPC results by overlapped injection using conventional size of columns and simultaneous determination of polymer additives.

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