Diffuse Reflectance Measurement

Diffuse Reflectance Measurement

Since light cannot penetrate opaque (solid) samples, it is reflected on the surface of the samples. As shown in the figure, incident light reflected symmetrically with respect to the normal line is called "specular reflection," while incident light scattered in different directions is called "diffuse reflection." 

 

Measurement of Diffuse Reflection Not Including Specular Reflection  Using an Integrating Sphere

Measurement of Diffuse Reflection Including Specular Reflection Using an Integrating Sphere

 
Reflection Spectrum of Paint Film

With integrating spheres, measurement is performed by placing the sample in front of the incident light window, and concentrating the light reflected from the sample on the detector using a sphere with a barium sulfate-coated inside. The obtained value becomes the reflectance (relative reflectance) with respect to the reflectance of the reference standard white board, which is taken to be 100%.
When light is directed at the sample at an angle of 0°, specular reflected light exits the integrating sphere and is not detected. As a result, only diffuse reflected light is measured. Models of integrating spheres with different angles of incidence are available, enabling measurement of both specular and diffuse reflected light in instances such as these.

Shimadzu Integrating Spheres for all UV-Vis spectrometers can measure both diffuse and spectral reflectance without using special attachments. This can be achieved by simply combining a 0°/8° incident integrating sphere with the S/R exchange function of the software. Smaller samples (few millimeters) can also be analyzed by reducing the flux of the incident beam.

Additionally, Shimadzu Integrating Spheres for UV-Vis spectrometers are also capable of measuring diffuse (and total) transmittance of solid, liquids and powders. Shimadzu Integrating Sphere for Fluorescence spectrometer allows for Fluorescence Quantum Yield and Fluorescence Quantum Efficiency measurements.

ISR-2600/ISR-2600Plus Integrating Sphere Attachment (60mm)

ISR-2600/ISR-2600Plus Integrating Sphere Attachment
  • Angle of incident light: 0°
  • Wavelength range:
    ISR-2600 220 to 850 nm, ISR-2600Plus 220 to 1400nm (UV-2600 Plus)
  • Maximum size of reflection sample:
    95 x 135 x 20 (W x H x T) mm
  • Cell holder for transmission sample: film holder, cuvette holder
  • ISR-2600 For: UV-2600 and UV-2700 UV-Vis spectrometers
    ISR-2600Plus For: UV-2600 spectrometer
 
Specular Reflectance Measurement is also possible (8° angle) 

ISR-603 Integrating Sphere Attachment (60 mm)

ISR-603 Integrating Sphere Attachment
  • Angle of incident light: 0°
  • Wavelength range: 220 to 2600 nm
  • Maximum size of reflection sample: 70 x 70 x 20 (W x H x T) mm
  • Cell holder for transmission sample: film holder, cuvette holder
  • For use with the UV-3600Plus
 
Specular Reflectance Measurement is also possible (8° angle)​

ISR-1503 Integrating Sphere Attachment (150mm)

ISR-1503 Integrating Sphere Attachment
  • Angle of incident light: 0°
  • Wavelength range: 200 to 2500 nm
  • Maximum size of reflection sample: 150 x 165 x 10 (W x H x T) mm
  • Cell holder for transmission sample: Film holder
  • For use with the UV-3600Plus
  • Specular Reflectance Measurement is also possible (8° angle)
 
Note: The optional cuvette attachment is required for transmission measurement of liquid samples. 

ISR-100 Fluorescence Integrating Sphere (100 mm)

ISR-100 Integrating Sphere Attachment
  • Angle of incident light: 0°
  • Wavelength range: 200 to 900 nm
  • Maximum size of reflection sample: 12.5 x 12.5 x 45 (W x D x H) mm
  • Maximum size of solid reflection sample: 35 – 58 mm (H), 3 – 12 mm (T)
  • For use with the RF-6000
  • Measurements: Fluorescence Quantum Yield, Fluorescence Quantum Efficiency
 

For Research Use Only. Not for use in diagnostic procedures.

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