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Quantification and Simultaneous In-Source Fragmentation of 16 Cannabinoids in Hemp Using Single Quadrupole LC-MS

Quantification and Simultaneous In-Source Fragmentation of 16 Cannabinoids in Hemp Using Single Quadrupole LC-MS

In this extended study, an LC-MS method of quantitation and simultaneous SID was developed using the LCMS-2020 single quadrupole MS with an integrated LC front end (LC 2040C 3D) for the quantification of 16 cannabinoids, including Δ9-tetrahydrocannabinol (Δ9-THC), Δ8-tetrahydrocannabinol (Δ8-THC), cannabidiol (CBD), cannabigerol (CBG), cannabinol (CBN), cannabicyclol (CBL) and their respective acidic forms, etc. Selected ion monitoring (SIM) was used for quantitation, simultaneous positive and negative scans were used for identification.


Tocopherol (Vitamin E) Analysis in Vaping E-Liquid by UHPLC-PDA

Tocopherol (Vitamin E) Analysis in Vaping E-Liquid by UHPLC-PDA

Vaping illnesses have been a growing concern for the CDC with over 2,000 known cases and 39 confirmed deaths so far (CDC – November 2019). It is unclear what is causing the illnesses, but vitamin E acetate has been identified as a possible contributor. Vitamin E acetate has been found in the bronchoalveolar lavage (BAL) fluid for all currently tested samples by the CDC (CDC – November 2019). Two methods have been developed using photodiode array detection (PDA) to separate three isomers of tocopherol (vitamin E): alpha-tocopherol acetate, alpha-tocopherol, and gamma-tocopherol.


In-Source Fragmentation of 16 Cannabinoids Using Single Quadrupole LC-MS

In-Source Fragmentation of 16 Cannabinoids Using Single Quadrupole LC-MS

For this study, an IS-CID method was developed using a single quadrupole MS with an integrated LC front end for chromatographic separation for 16 cannabinoids, including Δ9-tetrahydrocannabinol (Δ9-THC), Δ8-tetrahydrocannabinol (Δ8-THC), cannabidiol (CBD), cannabigerol (CBG), cannabinol (CBN), cannabicyclol (CBL), and their respective acidic forms etc. Both positive and negative scans and selected ion monitoring (SIM) were used simultaneously in the method and a MS library for the 16 cannabinoids was created for the rapid identification of target analytes in unknow samples.


Quantitative Analysis of 16 Cannabinoids with Complete Baseline Separation of Δ8 and Δ9-THC Utilizing the Triple Quad LCMS-8050

Quantitative Analysis of 16 Cannabinoids with Complete Baseline Separation of Δ8 and Δ9-THC Utilizing the Triple Quad LCMS-8050

The following method application is driven by the potential terpenes interferences with THC quantitation when analyzing fortified Cannabis products on an HPLC system with a UV or PDA detector. Generally, terpene interference is not a problem as naturally occurring terpenoid content does not exceed 0.2% of the total and terpenes are lost in the baseline under the currently run gradient conditions with HPLC-UV or UHPLC-PDA. However, a sample that has been fortified with excess terpene content may face co-elution issues of the terpenes with individual cannabinoids because the terpenes are also quite hydrophobic and exhibit similar retention on the column as the cannabinoids.


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Forensics Application Guidebook     Shimadzu Journal Vol 6 - Forensics/Toxicology and more...
 

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Forensic Webinar - Thermal Desorption GCMS: Supporting your Arson Analysis

Title: Thermal Desorption GCMS: Supporting Your Arson Analysis

Duration: 1 hour

 

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Automating Sample Preparation for LCMS with Shimadzu's CLAM-2000 Series
Shimadzu's Smart Forensic Database Enables Fast, Accurate Analysis of Toxicological Substances in whole blood
Analysis of Blood Alcohol by Headspace with Simultaneous GC-FID and MS Detection

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Streamlined, Single Method Detection of Fentanyl & Related Synthetic Opioids using Shimadzu's LCMS-8060 Video

Shimadzu Solutions for THC Quantitation Video

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