Perfinity Integrated Digestion Platform (iDP)

Rapid, Reproducible Protein Digestions

1-4 minutes trypsin digestion using a high-efficiency trypsin column

digestion of insulin

The benefits of using Perfinity iDP with the immobilized trypsin column are:

  1. Fast trypsin digestion in 1 to 4 minutes
  2. Continuous sample analysis on reusable trypsin column
  3. Reduced chymotrypsin activity and deamidation
  4. Standardization of the digestion process

Insulin was digested within 1 minute using the trypsin column as shown in the figure at right.
* Optimal digestion time depends on proteins.


Optimized Perfinity iDP digestion buffer

increased digestion efficiency by the perfinity idp digestion buffer

The optimized trypsin digestion buffer in combination with the trypsin column enables highly efficient digestion.
The figure to the right shows a comparison between the buffer. The optimized digestion buffer provided a higher peptide recovery rate with a short digestion time.


Increased throughput by parallel sample processing with two desalting columns

parallel process by two desalting columns

Two desalting columns allow parallel processing of samples. One desalting column is used to trap digested peptides, while peptides from the previously injected sample are eluted from the other desalting column. This parallel sample processing allows analysis of up to 200 samples/day

The figure to the right is an example of parallel sample processing, which increases throughput while maintaining reproducibility.

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