Automatic Sprayer for MALDI Imaging - iMLayer AERO 

Easily Obtain Images with High Sensitivity and High Spatial Resolution

iMLayer AERO Automatic Sprayer for MALDI Imaging

The iMLayer™ AERO, an automated matrix sprayer, incorporates a sample stage that moves at a controlled rate while maintaining the same distance from the spray nozzle, enabling stable matrix spraying.

Over multiple strokes, the sample becomes laminated with fine matrix crystals, enabling high sensitivity and high spatial resolution.

 

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System Highlights

System components and highlights

Features

Imaging with High Reproducibility

iMLayer AERO humidity control

Humidity Control

Humidity does not impact matrix deposition because the atmosphere within the spray chamber is replaced before pretreatment. Spraying is performed under controlled conditions which leads to more reproducible results. 

 
Clog-free Reagent Delivery

Clog-Free Reagent Delivery

If the matrix clogs the nozzle tip, the spray becomes unstable, which can lead to lower reproducibility. With the iMLayer AERO, a rinsing mechanism allows for clog-free, stable spraying, which leads to highly reproducible matrix spraying. 

Both Sensitivity and Spatial Resolution Achieved

Two-Step Vapor Deposition

Excellent Spray Performance

The standard protocol provides sensitivity on par with a hand spray along with improved resolution, homogeneity and reproducibility

 

Two-Step Vapor Deposition

To acquire high spatial resolution (5 to 10 μm) images and increase sensitivity, a two-step vapor deposition method can be implemented in combination with the iMLayer matrix vapor deposition system.* This unique experiment can only be implemented using Shimadzu sample preparation solutions.

* Patent No.: JP6153139 and JP6183779

 

Two-Step Vapor Deposition Allows for Very Fine Images with Minimal Blur

 
Hand Spray

Hand Spray

  • Matrix:9-AA
  • Volume used:200 μL
Two-Step Vapor Deposition

Two-Step Vapor Deposition

First Step:iMLayer(vapor deposition method)

  • Film thickness:1 μm
 

Second Step:iMLayer AERO(spray method)

 
  • Solution delivery volume:120 μL/min
  • Stage speed:70 mm/sec
  • Laminating layers:4
 

Applicable to Pretreatment Other than Matrix Coatings

Before and After Derivatization

Derivatization Treatment

iMLayer AERO is compatible with derivatization treatment. This option enables users to automate matrix deposition and chemical derivatization. In addition, iMlayer AERO users will have access to compounds that are difficult to ionize via MALDI. 

For Research Use Only. Not for use in diagnostic procedures.

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