Nexera X4 Ultra High-Performance Liquid Chromatograph - Specs

Ultra High-Performance Liquid Chromatograph

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Engineering Excellence

Innovative design pursuing maximum performance

Nexera X4 UHPLC System Components

Exhibiting Maximum Performance while Maintaining Instrument Robustness

  • Nexera X4 systems incorporate proprietary Nexflow technology that minimizes extra-column band broadening without changing the tube’s internal diameter or length. By reducing the risk of blockages and minimizing peak broadening, the Nexera X4 delivers outstanding separation performance.
  • Nexflow technology that minimizes extra-column band broadening without changing the tube’s internal diameter or length

  • Solvent Delivery Unit Achieves High-Speed High-Precision Gradients

    The LC-40B X4 is a binary gradient pump featuring four independently actuated plungers and a pressure feedback mechanism. Independent plunger actuation greatly reduces pump-derived pulsation and improves gradient responsiveness. These advanced fluid control technologies deliver stable baselines for ultra-fast gradients and consistent, reliable performance under high-pressure conditions.

  • High Responsiveness During Gradient Elution, Reduced Baseline Pulsation

  • Mobile Phase Switching and Degassing Unit Broadens the Analysis Width

    The SSU-40 is a solvent switching and online degassing unit for binary pumps that can switch between up to 4 solutions per pump. Automatic mobile phase switching helps improve the efficiency of method screening.

  • Solvent switching valve, online degassing unit

  • Newly Designed Low-Dispersion, High-Sensitivity PDA Detector

    The SPD-M40 X4 is a photodiode array detector that uses a capillary cell to reduce peak broadening. This design minimizes solvent-derived refractive index effects, leading to stable baselines and higher sensitivity. In addition, the broader dynamic range enables analysis of a wider range of low to high-concentration samples.

  • Improved baseline stability, improved linearity in high concentration area

  • High-End Autosampler Reduces Peak Broadening and Carryover

    High-End Autosampler Reduces Peak Broadening and Carryover
    The SIL-40C X4 is a high-end autosampler featuring a newly designed low-internal-volume high-pressure valve, low-dispersion injection mode, and high-speed needle internal/external rinsing. These capabilities make the SIL-40C X4 suitable for a wide range of samples, from those with extremely low concentrations that require high sensitivity to samples with high concentrations that require rinsing functionality.

  • Easy-to-Use Fitting Achieves Low-Dispersion Performance

    Easy-to-Use Fitting Achieves Low-Dispersion Performance
    The newly developed "Nexlock™ II" employs an end-surface sealing structure that eliminates ferrules and minimizes dead volume in tubing connections. Its proprietary fitting design also allows tool-free column connections while maintaining high-pressure tolerance.

 

Specifications

Solvent Delivery Unit

  LC-40B X4
LC-40B X4 Solvent Delivery Unit Pumping method Micro-volume double plunger pump
Allowable maximum pressure 130 MPa
Flow rate settings range 0.0001 to 2.0000 mL/min
Flow rate accuracy ±1 % (at 1 mL/min, 80 MPa, with water, stable within a temperature range of 20 to 30 °C)
Flow rate precision Retention time reproducibility:  ≤ 0.04 % RSD or ≤  0.003 min SD, whichever is greater
Gradient mode Two-solvent high-pressure gradient
Range of gradient mixing ratio 0 to 100 % (0.1 % step)
Gradient concentration precision ≤  0.1% RSD or ≤  0.008 min SD, whichever is greater (under specified conditions)
Wetted materials SUS316L, Co-Ni alloy, PEEK, ruby, sapphire, zirconia, Hastelloy C, polyethylene, fluoropolymer
Available pH range 1 to 14
Automatic rinsing kit Included as standard
Degassing unit 1 unit connectable
Dimensions and weight W260 × D500 × H210 mm (excluding protrusions), 24 kg
Operating temperature range 4 to 35 °C
Power supply 100 to 240 V AC, 50/60 Hz, 150 VA

Solvent Switching and Degassing Unit

  SSU-40
SSU-40 Solvent Switching and Degassing Unit

 

 Number of selectable
solvents		 Up to 4 solvents per pump (maximum 2 pumps)
Number of degassed
solvent lines		 5
Wetted materials Fluoropolymer, PEEK, PTFE, FEP, ETFE, perfluoroelastomer
Degassed flow line
capacity		 400 μL/1 line
Dimensions and weight W260 × D500 × H72 mm, 5 kg
Operating temperature
range		 4 to 35 °C
Power supply Supplied from the corresponding solvent delivery unit

Autosampler

  SIL-40C X4
SIL-40C X4 Autosampler

 

 Injection method Total-volume injection
Allowable maximum　pressure 130 MPa
Injection volume settings range 0.01 to 50 μL
Injection volume accuracy Within ±1% (5 μL injection, n = 20)
Linearity ≥ 0.9999
Injection cycle time ≤  6.7 seconds (under specified conditions)
Number of samples processed 288 (microtiter plate, 96 well × 3 plates)
1152 (microtiter plate, 384 well × 3 plates)
252 (1 mL sample vial, 84 × 3 plates)
162 (1.5 mL sample vial, 54 × 3 plates)
84 (4 mL sample vial, 28 × 3 plates)
36 (10 mL sample vial, 12 × 3 plates)
72 (1.5 mL micro tube, 24 × 3 plates)
Injection volume reproducibility RSD ≤  1.0% (0.5 to 0.9 μL)
RSD ≤  0.5% (1.0 to 1.9 μL)
RSD ≤  0.25% (2.0 to 4.9 μL)
RSD ≤  0.15% (more than 5.0 μL)
Cross-contamination ≤  0.0010 % (without rinse)
≤  0.0003 % (with rinse, typically)
(under specified conditions)
Dip rinsing outside the needle and injection port rinsing Included as standard
External needle flushing Included as standard
Internal needle flushing (3 solvents) Included as standard
Sample cooler Included as standard (air-circulation temperature control type)
Sample cooler temperature setting range 4 to 45 °C
(To set 4 °C, room temperature needs to be below 30 °C and humidity below 70 %)
Sample cooler temperature accuracy ±2 °C (sensor position ±0.5 °C)
Wetted materials Mobile phase flow line: SUS316L, DLC, PEEK
Other flow lines: SUS316L, PTFE, ETFE, FEP, GFP, sapphire, PPS, FEKM
Available pH range 1 to 14
Dimensions and weight W260 × D500 × H280 mm (protrusion adds 140 mm to the depth), 24 kg
Operating temperature range 4 to 35 °C
Power supply 100 to 240 V AC, 50/60 Hz, 400 VA

Plate Changer

  PLATE CHANGER
Plate Changer

 

 Number of samples processed
(including 2 plates of autosampler)		 Using 1 plate changer:
1536 (microtiter plate, 96 well × 16 plates)
864 (deep-well plate, 96 well × 9 plates)
6144 (microtiter plate, 384 well × 16 plates)
3456 (deep-well plate, 384 well × 9 plates)
756 (1 mL sample vial, 84 × 9 plates)
486 (1.5 mL sample vial, 54 × 9 plates)
252 (4 mL sample vial, 28 × 9 plates)
108 (10 mL sample vial, 12 × 9 plates)		 Using 3 plate changer:
4224 (microtiter plate, 96 well × 44 plates)
2208 (deep-well plate, 96 well × 23 plates)
16896 (microtiter plate, 384 well × 44 plates)
8832 (deep-well plate, 384 well × 23 plates)
1932 (1 mL sample vial, 84 × 23 plates)
1242 (1.5 mL sample vial, 54 × 23 plates)
644 (4 mL sample vial, 28 × 23 plates)
276 (10 mL sample vial, 12 × 23 plates)
Sample cooler Air-circulation temperature control type, 4 to 45 °C
(To set 4 °C, room temperature needs to be below 30 °C and humidity below 70 %)
Dimensions and weight W170 × D500 × H560 mm (protrusion adds 140 mm to the depth), 26 kg
Operating temperature
range		 4 to 35 °C
Dimensions and weight W260 × D500 × H72 mm, 5 kg
Operating temperature range 4 to 35 °C
Power supply (for 1 unit) 100 to 240 V AC, 400 VA, 50/60 Hz

Column Oven

    CTO-40C CTO-40S
CTO-40C and CTO-40S Column Ovens

 

 Temperature control type Forced air circulation
Cooling method Electronic cooling
Temperature control range Room temperature—10 to 100 °C Room temperature—10 to 85 °C
Temperature accuracy ±0.5 °C ±0.8 °C
Temperature precision 0.1 °C (±0.05 °C) 0.2 °C (±0.1 °C)
Containable column size and number 6 columns, each up to 250 mm long,
or 3 columns, each up to 300 mm long		 6 columns, each up to 100 mm long,
or 3 columns, each up to 300 mm long
Dimensions and weight W260 × D500 × H415 mm, 21 kg W130 × D500 × H553 mm, 15 kg
Operating temperature range 4 to 35°C
Power supply 100 to 120 V AC/220 to 240 V AC
(automatic switching)
400 VA, 50/60 Hz		 100 to 240 V AC, 300 VA, 50/60 Hz

PDA Detector

  SPD-M40 X4
SPD-M40 X4 PDA Detector

 

 Light source D2 lamp
Number of photodiode array elements 1024
Wavelength range 190 to 800 nm
Wavelength accuracy ≤  ±1 nm
Wavelength reproducibility ≤ ±0.1 nm
Slit width 1.2 nm, 8 nm
Spectral resolution ≤  1.4 nm
Drift ≤ 0.5 × 10-3 of AU/h (under specified conditions)
Noise ≤ 4.0 × 10-6 AU (under specified conditions)
Linearity 3.0 AU (under specified conditions, typically)
Flow cell Optical path length: 10 mm, Cell volume: 0.5 μL, Maximum pressure: 8 MPa
Material of wetted parts: PEEK, PCTFE, PFA, quartz
Sampling rate Max. 250 Hz
Optional flow cell High-sensitivity cell
(Optical path length: 85 mm, Cell volume: 4.2 μL, Maximum pressure: 8 MPa)
Available pH range 1 to 13 (Depending on the type of mobile phase, if used for a long period at pH 10 or higher,
the quartz of the flow cell may be damaged, causing changes in transmission properties.)
Dimensions and weight W260 × D500 × H140 mm, 10 kg
Operating temperature range 4 to 35 °C
Power supply 100 to 240 V AC, 180 VA, 50/60 Hz
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