i-Series Integrated HPLC and UHPLC Systems - Specs

High Performance Liquid Chromatograph

LC-2050C (UV model)

Pump

Degassing unit	Five Lines: Mobile phase 4 + Rinse solution 1 [Volume 400 μL]
Pumping method	Parallel-type double plunger
Pulsation	≤ 0.1 MPa [1.0 mL/min,10 MPa, Water]
Flow rate setting range	0.0001 to 10 mL/min
Flow rate accuracy	≤ ±1% or ≤ ±2 μL/min, below whichever is greater [0.01 to 2 mL/min, Specified condition] ≤ ±2% [2 to 5 mL/min, Specified condition]
Flow rate precision	< 0.06 %RSD or < 0.02 minSD, below whichever is greater
Configuration	Four-solvent low-pressure gradient
Gradient / range of set concentrations	0 to 100%, in 0.1% steps
Gradient / concentration accuracy	±0.5% [0.1 to 2 mL/min, 1 to 20 MPa, Specified condition]
Gradient / concentration precision	±0.1% [1 mL/min,10 MPa, Specified condition]
Maximum pressure	50 MPa [0.0001 to 5 mL/min] 22 MPa [5.0001 to 10 mL/min]
System Delay Volume	650 μL [Option: 460 μL, 1100 μL]

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Column Oven

Heating and cooling method	Forced air circulation method
Containable column size	6 pieces at 10 cm max., 3 pieces at 10 to 30 cm
Temperature control range	Room temperature − 12 to 90°C, Setting range 4 to 90°C
Temperature control precision	±0.1°C
Temperature stability	±0.8°C (Specified condition)
Flow rate switching valve	Max. 1 pc

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UV Detector

Wavelength range	190 to 700 nm
Spectral bandwidth	8 nm
Wavelength accuracy	≤ ±1 nm
Wavelength Reproducibility	≤ ±0.1 nm
Noise	≤ ±2.5 × 10−6 AU, (250 nm, Specified condition)
Drift	≤ 100 × 10−6 AU/h [250 nm, Specified condition]
Simultaneous monitoring of 2 wavelengths	Enable [Any 2 wavelengths of 190 to 370 nm or 371 to 700 nm]
Linearity	Up to 2.5 AU (5%)
Sampling rate	Up to 100 Hz
Light source	Deuterium (D2) lamp
Flow Cell	12 μL (10 mm, TC), 12 MPa
Option cell	High-Speed: 8 μL [10 mm, TC] Semi-micro: 2.5 μL [5mm, TC]

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Miscellaneous

Dimensions	W410×H605×D500 mm [Not including reservoir tray height]
Weight	63 kg
Available pH range	1 to 13
Materials for parts in contact with liquids	Stainless steel (SUS316L, SUS316), FEP, PEEK, PTFE, perfluoroelastomer, ruby, sapphire, Hastelloy® C, GFP, ceramic, PFA, quartz, PPS

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Autosampler

Sample injection method	Total-volume sample injection
Injection volume accuracy	±1% [50 μL, N = 10]
Injection volume setting range	0.1 to 100 μL [Option: 0.1 to 50 μL, 1 to 500 μL, 1 to 2,000 μL]
Injection volume reproducibility	RSD < 0.20% [5.0−2000 μL] RSD < 0.25% [2.0−4.9 μL] RSD < 0.5% [1.0−1.9 μL] RSD < 1.0% [0.5−0.9 μL]
Cross-contamination	0.0025% [Caffeine, Specified condition]
Injection cycle time	Min. 14 sec [Specified condition]
Samples for processing	336 [1 mL], 216 [1.5 mL], 112 [4 mL], 4 [MTP/DWP]
Sample cooler	4 to 45°C [Room temperature needs to be 30°C or lower and the humidity 70% or less to go down to 4°C. Room temperature needs to be 15°C or higher to go up to 45°C.]
Injection linearity	> 0.9999 [1 to 100 μL, Specified condition]

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