Nexera Analytical SFC - Configuration

Supercritical Fluid Chromatography System

System configuration examples

SFC-UV / SFC-MS System

SFC-UV / SFC-MS System

This is the minimum setup of Nexera UC and can replace both normal phase and reverse phase HPLCs. A wide range of analyte polarity can be covered by the combination of supercritical CO2 and modifiers (for example, MeOH). Hazardous organic solvents such as hexane or chloroform are eliminated. In addition, the system reduces environmental impact by utilizing low-toxicity mobile phases and completing analyses in a shorter time. Both UV-Vis and MS detectors such as the triple quad LCMS-8050 can be successfully coupled with this SFC system.

Chiral Screening System

Chiral Screening System

This system is best for developing methods to separate chiral compounds. It automatically generates a large number of methods by utilizing combinations of up to 12 columns, four modifiers, and a different ratio of modifiers to mobile phase.

UHPLC / SFC Switching system : Nexera UCs

UHPLC / SFC Switching system : Nexera UCs

This system can switch automatically between SFC analysis and UHPLC analysis and make measurements on a single sample in each separation mode. It enhances user-friendliness and operability by allowing the investigation of separation conditions and performing reverse-phase high-speed analysis in a single system. Shimadzu also provides a kit to upgrade from your current UHPLC system to the UHPLC/SFC switching system.

Analytical Fraction System : analytical flow and fraction collection in one system

Analytical Fraction System : analytical flow and fraction collection in one system

This system is intended for analytical scale fractionation only requiring fraction volumes of several mL to recover up to 20 mg of material (used to check synthesis for example). By connecting an FRC-40 SF fraction collector to the Nexera UC system, analytical columns from 2.1 to 4.6 mm I.D. can be run at flow rates up to 5 mL/min for small volume fraction collection.

On-line SFE-SFC-MS System

On-line SFE-SFC-MS System

In this system, solid samples are extracted by supercritical COand introduced to SFC on-line. The time for pretreatment of samples is drastically shortened. In addition, samples are extracted under light-shielding and anaerobic conditions to protect labile analytes from degradation. By adding a fraction collector to this system, small-volume fractionation of the extract is also available.

SFE Pretreatment System

SFE Pretreatment System

This system allows the pretreatment of samples using supercritical CO2. An extraction operation that changes the types of modifiers (up to four types) and concentrations to mix with supercritical COcan be performed on each sample. The extracted material is collected in a test tube using a FRC-40 SF fraction collector. The LotusStream separator allows fractionation of small volumes, even into vessels such as 1.5 mL vials, without dispersion of the solvent. In addition to analysis by SFC, the system is ideal for measurements using other analytical systems such as GC-MS and NMR.