PPSQ-51A/53A Protein Sequencers

Isocratic System


PPSQ-51A/53A Protein Sequencer Isocratic System

Analysis of PTH-Amino Acids Using Isocratic Mode

Performing PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs.

Simple Operations

Specialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis.


Analytical Stability and Higher Detection

Baseline Stability

PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.

Retention Time Reproducibility

Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.

Analytical Stability and Higher Detection - PTH-AA 10pmol

Higher Detection

Cycle 10 PTH-Val

PPSQ-51A/53A Cycle 10 PTH-Val


PPSQ-31B/33B Cycle 10 PTH-Val


Cycle 21 PTH-Ile

PPSQ-51A/53A Cycle 21 PTH-Ile


PPSQ-31B/33B Cycle 21 PTH-Ile


Sample: Horse myoglobin 10pmol
Results show the subtraction chromatograms by SPD-M30A (PPSQ-51A/53A) and SPD-20A (PPSQ-31B/33B) connected tandemly after Edman degradation.


Main Unit PPSQ-51A/53A
Reaction method Edman degradation
Reaction time PPSQ-51A: 46.5 min/cycle
PPSQ-53A: 48 min/cycle
Number of reactors PPSQ-51A: 1
PPSQ-53A: 3
Sample immobilization method Glass fiber disk (8 mm dia.) or PVDF membrane
Reactor temperature control range 10 °C above room temperature to 60 °C
Converter temperature control range 10 °C above room temperature to 70 °C
Number of reagents/solvents 7
Reagent/solvent delivery method Nitrogen gas pressure
Dimensions W510 x D500 x H540 mm
Weight PPSQ-51A: 43 kg
PPSQ-53A: 45 kg
Solvent Delivery Module LC-20AT
Flow rate setting range 0.001 to 10 mL/min
Dimensions W260 x D420 x H140 mm
Weight 11 kg
Detector SPD-M30A
Wavelength setting range 190 to 700 nm
Dimensions W260 x D500 x H140 mm
Weight 12 kg
Oven CTO-20A
Temperature control range 10 °C above room temperature to 60 °C
Dimensions W260 x D420 x H415 mm
Weight 20 kg
Control PC
OS Windows 10 Pro 64 bit

Note: In the interests of product improvement, these specifications may change without notice.
Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries.

Standard Configurations

PPSQ-51A System
Configuration PPSQ-51A (main unit)

PPSQ-53A System
Configuration PPSQ-53A (main unit)

Other Items Provided by User

Installation space Desktop: W1,600 x D600 x H800 mm min.
Weight: Approx. 100 kg
Power supply 120-230 VAC, 50/60 Hz, 1,500 VA max.
Nitrogen gas Purity: 99.9999% min.
A cylinder pressure regulator and gas tubing (10 m) are provided as standard accessories.
Exhaust equipment In order to provide an exhaust for gases produced by waste liquids, an exhaust tube must either be connected to exhaust equipment or vented outside. An exhaust tube (20 m) is provided as a standard accessory.

Note: The required installation space and power supply may change according to the PC and display used.

Installation Example

PPSQ-51A/53A Protein Sequencer Isocratic System Installation Example

Unit: mm

PPSQ-51A Single Reactor

PPSQ-51A Single Reactor

Offers highly cost-effective performance.

PPSQ-53A Triple Reactor (Supports consecutive analysis of 3 samples.)

PPSQ-53A Triple Reactor (Supports consecutive analysis of 3 samples.)

Using a triple reactor makes it possible to save time and effort, and gives greater freedom in the formulation of analysis programs.

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