PPSQ-51A/53A Protein Sequencers
Analysis of PTH-Amino Acids Using Isocratic Mode
Performing PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs.
Specialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis.
Analytical Stability and Higher Detection
PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.
Retention Time Reproducibility
Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.
Sample: Horse myoglobin 10pmol
Results show the subtraction chromatograms by SPD-M30A (PPSQ-51A/53A) and SPD-20A (PPSQ-31B/33B) connected tandemly after Edman degradation.
|Reaction method||Edman degradation|
|Reaction time||PPSQ-51A: 46.5 min/cycle|
|PPSQ-53A: 48 min/cycle|
|Number of reactors||PPSQ-51A: 1|
|Sample immobilization method||Glass fiber disk (8 mm dia.) or PVDF membrane|
|Reactor temperature control range||10 °C above room temperature to 60 °C|
|Converter temperature control range||10 °C above room temperature to 70 °C|
|Number of reagents/solvents||7|
|Reagent/solvent delivery method||Nitrogen gas pressure|
|Dimensions||W510 x D500 x H540 mm|
|Weight||PPSQ-51A: 43 kg
PPSQ-53A: 45 kg
|Solvent Delivery Module||LC-20AT|
|Flow rate setting range||0.001 to 10 mL/min|
|Dimensions||W260 x D420 x H140 mm|
|Wavelength setting range||190 to 700 nm|
|Dimensions||W260 x D500 x H140 mm|
|Temperature control range||10 °C above room temperature to 60 °C|
|Dimensions||W260 x D420 x H415 mm|
|OS||Windows 10 Pro 64 bit|
Note: In the interests of product improvement, these specifications may change without notice.
Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries.
|Configuration||PPSQ-51A (main unit)|
|Configuration||PPSQ-53A (main unit)|
Other Items Provided by User
|Installation space||Desktop: W1,600 x D600 x H800 mm min.|
|Weight: Approx. 100 kg|
|Power supply||120-230 VAC, 50/60 Hz, 1,500 VA max.|
|Nitrogen gas||Purity: 99.9999% min.|
|A cylinder pressure regulator and gas tubing (10 m) are provided as standard accessories.|
|Exhaust equipment||In order to provide an exhaust for gases produced by waste liquids, an exhaust tube must either be connected to exhaust equipment or vented outside. An exhaust tube (20 m) is provided as a standard accessory.|
Note: The required installation space and power supply may change according to the PC and display used.