UV-1280 UV-Vis Spectrophotometer

Multipurpose UV-Visible Spectrophotometer

UV-1280 Single Beam UV-Vis Spectrophotometer

The Shimadzu UV-1280 is a low-cost, high-quality instrument ideal for applications ranging from routine environmental and food quality testing to life science analyses. The monitored double-beam UV-1280 is equipped with seven measurement modes, features USB data portability, and offers wavelength scanning from 190 to 1,100 nm. Combining these features with intuitive operation improves work efficiency, making the compact UV-1280 the ideal choice for applications in a variety of industries.

The instrument features a keypad with a LED screen to enable user-friendly intuitive measurements, instrument validation, and printing operations. Measurement modes include photometric, spectrum, quantitation, kinetics, time course and bio-methods. Additionally, with the optional software, Shimadzu VisEaseTM, designed as a simplified application program with minimum interface, the UV-1280 can be operated with a PC.

UV-1280 Features

  • Easy-to-see LCD and buttons enable user-friendly intuitive measurements, instrument validation and printing operations
  • Built-in validation to check the integrity of the instrument and data. Features semi-automatic mode and automatic mode for detailed and quick validations respectively
  • Multi-cell changer and accessories for temperature-controlled measurements are available
  • Data storage on USB drive from the spectrophotometer without the use of a PC

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A Variety of High-Level Measurement Modes

Equipped with a range of programs, the UV-1280 can be used for everything from photometric, spectral, and kinetics measurements to DNA/protein and high-level multi-component quantitation.

Photometric Mode Measures the absorbance or transmittance at a single wavelength or multiple (up to eight) wavelengths. The instrument is also capable of simple quantitation using the K-factor method. For a multiple-wavelength measurement, calculations can be performed on the data obtained for up to four wavelengths, including the calculation of the difference between, or ratio of, the measurements obtained for two wavelengths.
Spectrum Mode A sample spectrum is recorded using wavelength scanning. Repeat scans let you follow sample changes over time. Zoom in on the finished spectrum for a better view; subsequently, use the peak/valley pick function to select maxima and minima and perform a wide variety of data processing functions.
Quantitation Mode Generates a calibration curve from the measurement of standards, and then calculates the concentrations of unknowns. Allows various combinations of wavelength number (1 to 3 wavelengths and derivatives) and calibration curves (K-factor and first-to-third order).
Kinetics Mode Measures absorbance changes as a function of time, and obtains the enzymatic activity values. The kinetics measurement method automatically calculates the amount of change per minute, and then calculates an activity value from a specified coefficient. The rate measurement method, which determines whether the absorbance is changing linearly, can also be selected. In addition, add the CPS-100 thermoelectrically temperature-controlled cell positioner for measurement of multiple samples in succession.
Time Scan Mode Measures the change in absorbance, transmittance or energy as a function of time. Add the CPS-100 thermoelectrically temperature-controlled cell positioner for simultaneous measurement of multiple samples under constant-temperature conditions.
Quantitation Mode
Quantitates up to eight components mixed in a single sample. The calibration equation is determined using pure or mixed components with known values.
Biomethod Mode Determine DNA and protein concentrations with the following quantitation methods using the bioscience/life science program included as standard.

DNA/Protein Quantitation

  • Quantitates DNA or protein using the absorbance at 260/230 nm or 260/280 nm.

Protein Quantitation

  • Lowry method
  • BCA method (method using bicinchoninic acid)
  • Biuret method
  • CBB method (method using Coomassie Brilliant Blue G-250)
  • UV absorption method (direct measurement at 280 nm)

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Documents & Resources

Relevant Application Notes

Relevant Literature Citations

Hoshino, S., Ozeki, M., Awakawa, T., Hiroyuki, M., & Onaka, H. (2018). Catenulobactins A and B, Heterocyclic Peptides from Culturing Catenuloplanes sp. with a Mycolic Acid-Containing Bacterium. Journal of Natural Products, 2106-2110.  https://pubs.acs.org/doi/10.1021/acs.jnatprod.8b00261


Speckmeier, E., Klimkait, M., & Zeitler, K. (2018). Unlocking the Potential of Phencyl Protecting Groups: CO2-Based Formation and Photocatalytic Release of Caged Amines. Journal of Organic Chemistry, 3738-3745.  https://pubs.acs.org/doi/10.1021/acs.joc.8b00096


Sibalic, D., Salic, A., Zelic, B., Tran, N. N., Hessel, V., & Tisma, M. (2020). A New Spectrophotometric Assay for Measuring the Hydrolytic Activity of Lipase from Thermomyces lanuginosus: A Kinetic Modeling. ACS Sustainable Chemistry & Engineering, 4818-4826.  https://pubs.acs.org/doi/10.1021/acssuschemeng.9b07543


Okamoto, Y., Yasuda, T., Sumiya, M., & Suzuki, Y. (2018). Perovskite Solar Cells Prepared by Advanced Three-Step Method Using Additional HC(NH2)2I Spin-Coating: Efficiency Improvement with Multiple Bandgap Structure. ACS Applied Energy Materials, 1389-1394.  https://pubs.acs.org/doi/10.1021/acsaem.8b00178


Tierney, T., Bodnar, K., Rasmuson, A., & Hudson, S. (2017). Carrier particle design for stabilization and isolation of drug nanoparticles. International Journal of Pharmaceutics, 111-118.  https://www.sciencedirect.com/science/article/pii/S0378517316311085

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