UV-1280 UV-Vis Spectrophotometer
Multipurpose UV-Visible Spectrophotometer
The Shimadzu UV-1280 is a low-cost and high-quality instrument ideal for applications ranging from routine environmental and food quality testing to life science analyses. The monitored single-beam UV-1280 is equipped with seven measurement modes, features USB data portability, and offers wavelength scanning from 190 to 1,100 nm. Combining these features with intuitive operation improves work efficiency, making the compact UV-1280 the ideal choice for applications in a variety of industries.
The instrument features a keypad with a led screen to enable user-friendly intuitive measurements, instrument validation, and printing operations. Measurement modes include photometric, spectrum, quantitation, kinetics, time course and bio-methods. Additionally, with an optional software, Shimadzu VisEaseTM, designed as a simplified application program with minimum interface, the UV-1280 can be operated with a PC.
A Variety of High-Level Measurement Modes
Equipped with a range of programs, the UV-1280 can be used for everything from photometric, spectral, and kinetics measurements to DNA/protein and high-level multi-component quantitation.
|Photometric Mode||Measures the absorbance or transmittance at a single wavelength or multiple (up to eight) wavelengths. The instrument is also capable of simple quantitation using the K-factor method. For a multiple-wavelength measurement, calculations can be performed on the data obtained for up to four wavelengths, including the calculation of the difference between, or ratio of, the measurements obtained for two wavelengths.|
|Spectrum Mode||A sample spectrum is recorded using wavelength scanning. Repeat scans let you follow sample changes over time. Zoom in on the finished spectrum for a better view; subsequently, use the peak/valley pick function to select maxima and minima and perform a wide variety of data processing functions.|
|Quantitation Mode||Generates a calibration curve from the measurement of standards, and then calculates the concentrations of unknowns. Allows various combinations of wavelength number (1 to 3 wavelengths and derivatives) and calibration curves (K-factor and first-to-third order).|
|Kinetics Mode||Measures absorbance changes as a function of time, and obtains the enzymatic activity values. The kinetics measurement method automatically calculates the amount of change per minute, and then calculates an activity value from a specified coefficient. The rate measurement method, which determines whether the absorbance is changing linearly, can also be selected. In addition, add the CPS-100 thermoelectrically temperature-controlled cell positioner for measurement of multiple samples in succession.|
|Time Scan Mode||Measures the change in absorbance, transmittance or energy as a function of time. Add the CPS-100 thermoelectrically temperature-controlled cell positioner for simultaneous measurement of multiple samples under constant-temperature conditions.|
|Quantitates up to eight components mixed in a single sample. The calibration equation is determined using pure or mixed components with known values.|
|Biomethod Mode||Determine DNA and protein concentrations with the following quantitation methods using the bioscience/life science program included as standard.
- Easy-to-see LCD and buttons enable user-friendly intuitive measurements, instrument validation and printing operations
- Built-in validation to check the integrity of the instrument and data. Features semi-automatic mode and automatic mode for detailed and quick validations respectively
- Multiple measurement modes such as photometric, spectrum, quantitation, kinetics, time course and bio-methods
- Multi-cell changer and accessories for temperature-controlled measurements are available
- Data storage on USB drive from the spectrophotometer without the use of a PC
Documents & Resources
Relevant Application Notes
- Measurement of Hexavalent Chromium in Chromate Conversion Coating and Metal Ions in Eluate – Application of Water Analysis Program for the UV-1280
- Analysis of Mineral Water Hardness and Iron and Zinc Content of Supplements
- UV-1280, TrayCell, Nucleic Acid Micro Volume Photometric Measurement of Nucleic Acid using TrayCell with UV-1280
- Chromium (VI) analysis according to the RoHS guidelines - Hardware and software for accurate determination of hazardous substances
Relevant Literature Citations
Hoshino, S., Ozeki, M., Awakawa, T., Hiroyuki, M., & Onaka, H. (2018). Catenulobactins A and B, Heterocyclic Peptides from Culturing Catenuloplanes sp. with a Mycolic Acid-Containing Bacterium. Journal of Natural Products, 2106-2110. https://pubs.acs.org/doi/10.1021/acs.jnatprod.8b00261
Speckmeier, E., Klimkait, M., & Zeitler, K. (2018). Unlocking the Potential of Phencyl Protecting Groups: CO2-Based Formation and Photocatalytic Release of Caged Amines. Journal of Organic Chemistry, 3738-3745. https://pubs.acs.org/doi/10.1021/acs.joc.8b00096
Sibalic, D., Salic, A., Zelic, B., Tran, N. N., Hessel, V., & Tisma, M. (2020). A New Spectrophotometric Assay for Measuring the Hydrolytic Activity of Lipase from Thermomyces lanuginosus: A Kinetic Modeling. ACS Sustainable Chemistry & Engineering, 4818-4826. https://pubs.acs.org/doi/10.1021/acssuschemeng.9b07543
Okamoto, Y., Yasuda, T., Sumiya, M., & Suzuki, Y. (2018). Perovskite Solar Cells Prepared by Advanced Three-Step Method Using Additional HC(NH2)2I Spin-Coating: Efficiency Improvement with Multiple Bandgap Structure. ACS Applied Energy Materials, 1389-1394. https://pubs.acs.org/doi/10.1021/acsaem.8b00178
Tierney, T., Bodnar, K., Rasmuson, A., & Hudson, S. (2017). Carrier particle design for stabilization and isolation of drug nanoparticles. International Journal of Pharmaceutics, 111-118. https://www.sciencedirect.com/science/article/pii/S0378517316311085