RF-6000 Fluorescence Spectrofluorometer

Enabling Accurate, Efficient Fluorescence Spectroscopy Analysis

Fluorescence Webinar - An Introduction to Light Emission Spectroscopy, with a focus on Fluorescence: Theory and Materials Characterization Applications

RF-6000 Fluorescence Spectrofluorometer

Incorporating technology cultivated over decades of developing fluorescence spectrometers, the versatile RF-6000 fluorescence spectrophotometer maximizes the capabilities of fluorescence spectroscopy with high-speed 3D measurements, ultrafast scanning, outstanding sensitivity, and more uptime. When combined with intuitive software, the RF-6000 spectrofluorometer is ideal for a wide range of applications, from routine analysis to research and development, in such areas as pharmaceuticals, food science, environmental monitoring, and chemicals.

The RF-6000 fluorescence spectrophotometer offers an extended wavelength and outstanding sensitivity, in addition to a wealth of available accessories, to make it a perfect instrument for both traditional and research applications. In addition to conventional excitation-emission analysis, multiple spectral techniques are available for a variety of applications, such as bioluminescence, chemiluminescence and electro-luminescence. Furthermore, high-speed 3D scanning allows obtaining fluorescence excitation-emission measurements (FEEM) for rapid and accurate differentiation of very similar species. Fluorescence quantum yield and fluorescence quantum efficiency measurements (using the integrating sphere) are also available, along with the capability to analyze liquids, gels, solids, films and powder samples.

With the user’s concern in mind and incorporating years of innovation, the RF-6000 was developed to provide high sensitivity, stability, speed and reliability. It boasts the highest S/N in its class, a scanning speed of up to 60,000 nm/min, 2,000-hr long-life Xe lamp and an extended range PMT detector to scan the wavelength range up to 900 nm. Additionally, the built-in spectral-correction function of the RF-6000 yields ready-to-use accurate data without the need for cumbersome spectral-correction procedures. With all the advanced features, the RF-6000 is suitable for a wide variety of fields, including pharmaceuticals, chemicals, life science, food, environmental and electrical/electronics.

In addition, with the included advanced LabSolutions RF control software, users can get the most out of the instrument in terms of data acquisition, analysis, data integrity and user administration. With the proper software compliance package, the RF-6000 and the software setup can provide total data integrity, user administration and audit trails to fully comply with FDA 21 CFR Part 11 compliance.


RF-6000 Features

  • In addition to traditional excitation-emission analysis, multiple other analyses are available, such as bioluminescencechemiluminescence and electro-luminescence.
  • Fluorescence Excitation-Emission Matrix (FEEM) and Synchronous Scanning for differentiating between very similar species
  • High-speed scanning, up to 60,0000 nm/min, for very quick data acquisition
  • Quantum Yield and Quantum Efficiency
  • 2,000-hr long-life Xe lamp and Extended range PMT detector to scan the wavelength range up to 900 nm
  • Built-in spectral-correction function
  • Suitable for a wide variety of fields, including pharmaceuticals, chemicals, life science, food, environmental and electrical/electronics
  • Multitude of accessories available for analyzing liquids, gels, solids, films and powder samples. Accessories include a temperature-controlled cuvette holder, integrating sphere, solid sample holder, autosamplers, microsample holders and polarizers
  • Advanced Regulatory Compliance
    • GLP/GMP, FDA 21 CFR Part 11 and other regulations with the appropriate software package
    • Full support for Pharmacopeia (JP, USP and EP). Validation programs are included for users to periodically measure and record the integrity of measurements
  • Enhanced security functions to provide audit trails and various user authority levels (“Administrator”, “Developer” and “Operator”)

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Documents & Resources

Relevant Application Notes


Relevant Literature Citations

meen, F., Siddiqui, S., Jahan, I., Nayeem, S., Rehman, S., & Tabish, M. (2020). A detailed insight into the interaction of memantine with bovine serum albumin: A spectroscopic and computational approach, Journal of Molecular Liquids, 303, 112671. https://doi.org/10.1016/j.molliq.2020.112671


Jones, D., Point, B., & Levine, M. (2019). 1.Effects of Structural Variation in Conjugated Side Chains on the Photophysics of Conjugated Polymers in Nanoparticles. Journal of Physical Chemistry B, 123(21), 4604-4610. https://doi.org/10.1021/acs.jpcb.9b01033


Korrapati, S., Sanjeeva, S., Chandrappa, M., Bhaskaran Nair, C., Mangalore Kini, G., Rao, P., . . . Pullela, P. (2018). Reverse micelle formation in vegetable oil, 1-butanol and diesel biofuel blends – Elimination of need for transesterification of triglycerides. Renewable Energy Focus, 25, 57-64. https://doi.org/10.1016/j.ref.2018.03.001


Matencio, A., Navarro-Orcajada, S., Garcia-Carmona, F., & Lopez-Nicolas, J. (2018). Ellagic acid–borax fluorescence interaction: application for novel cyclodextrin-borax nanosensors for analyzing ellagic acid in food samples. Food & Function, 9, 3683-3687. https://doi.org/10.1039/C8FO00906F


Wang, F., Wang, X., Zhang, M., Huang, A., & Ma, L. (2018). Conformational change of lysozyme on the interaction with gene carrier polyethyleneimine. International Journal of Biological Macromolecules, 117, 532-537. https://doi.org/10.1016/j.ijbiomac.2018.05.194

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