Health, Dietary, Nutraceuticals, & Supplements
Simultaneous Analysis of Water-soluble and Fat-soluble Vitamins in Fish by Novel SFC-MS/MS Method
Vitamins, essential dietary nutrients, are principally classified into water-soluble and fat-soluble groups based upon the solubility. Multiple chromatographic systems are commonly employed to analyse water-soluble and fat-soluble vitamins separately as both groups exhibit different chemical properties . Supercritical fluid carbon dioxide (SF-CO2) has been extensively utilized as a common solvent due to its advantages including non-toxic, low viscosity, high diffusivity, and easily removed from the reservoir . Despite its nonpolar characteristic, the polarity of SF-CO2 can be easily attuned by adding polar solvents such as methanol and ethanol. With the use of SF-CO2 as the mobile phase, supercritical fluid chromatography - mass spectrometry (SFC-MS) has attracted much attention for separation and analysis of diverse compounds. By changing the composition of mobile phase from supercritical to near liquid phase with addition of polar modifier (e.g., MeOH) up to 90%, we develop a novel SFC-MS/MS method for simultaneous analysis of 17 water soluble and fat soluble vitamins in fish samples. A novel SFC-MS/MS method for quantitative analysis of vitamins in fish sample was developed using a Raptor Biphenyl SFC column. Simultaneous analysis of eight water-soluble vitamins (B1, B2, B5, B6, B7, B3 acid, B3 amide and B12) and nine fat-soluble vitamins (D2, D3, E, E acetate, E succinate, K1, K2, A and β-carotene) was achieved in 6.5 minute using a gradient supercritical fluid chromatographic program with MeOH as the modifier.
Simultaneous Analysis of Water-soluble and Fat-soluble Vitamins in Fish by Reversed-Phase LC-MS/MS Method
Vitamins are a group of compounds that are essential for maintaining good health. As most of them cannot be produced by human body, vitamins are obtained mainly from external sources such as fruits and fish. Analysis methods of HPLC-UV and LC-MS were reported for different types of vitamins. Fat-soluble vitamins are analysed normally using normalphase chromatography, while water-soluble ones by reversed-phase chromatography [1,2]. Fish is an important source for both fat-soluble vitamins (D and E) and water-soluble vitamins (B) . It is desired to develop methods enabling determination of both fat-soluble and water-soluble vitamins. We describe a novel LC/MS/MS method for simultaneous analysis of 17 fat-soluble and water-soluble vitamins, aiming at quantitation of total vitamins in fish samples using a single method.A quantitation method for simultaneous analysis of nine water-soluble vitamins (B1, B2, B5, B6, B7, B3 acid, B3 amide, B9 and B12) and eight fat-soluble vitamins (D2, D3, E, E acetate, E succinate, K1, K2 and A and) was developed by employing reversed-phase LC/MS/MS platform. This LC/MS/MS method demonstrated high sensitivity for quantitative profiling of vitamins in fish sample.