Veterinary Drugs

Determination of 17 Hormone Residues in Milk by Ultra-High-Performance Liquid Chromatography and Triple Quadrupole Mass Spectrometry
This application news presents a method for the determination of 17 hormone residues in milk using Shimadzu Ultra-High-Performance Liquid Chromatograph (UHPLC) LC-30A and Triple Quadrupole Mass Spectrometer LCMS-8040. After sample pretreatment, the compounds in the milk matrix were separated using UPLC LC-30A and analyzed via Triple Quadrupole Mass Spectrometer LCMS-8040.
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Determination of Five Tetracycline Antibiotics Residues in Fish by Ultra-High-Performance Liquid Chromatography coupled with Triple Quadrupole Mass Spectrometry
This application note describes a method for the determination of five tetracycline antibiotics residues in fish using Shimadzu Ultra-High-Performance Liquid Chromatography coupled with Triple Quadrupole Mass Spectrometry. After solid phase extraction, the tetracycline antibiotics in fish were rapidly separated using Ultra Performance Liquid Chromatograph LC-30A in 4 min. The compounds were quantitatively analyzed using Triple Quadrupole Mass Spectrometer LCMS-8040.
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Determination of Chlorpromazine and Diazepam in Pork by Ultra-High-Performance Liquid Chromatography coupled with Triple Quadrupole Mass Spectrometry
A method for the determination of chlorpromazine and diazepam in pork using Shimadzu Ultra-High-Performance Liquid Chromatograph LC-30A and Triple Quadrupole Mass Spectrometer LCMS-8040 was developed.
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Determination of Eight Tranquilizers in Pork by Ultra-High-Performance Liquid Chromatography coupled with Triple Quadrupole Mass Spectrometry
This application note presents a method for the determination of eight tranquilizers in pork using Shimadzu Ultra-High-Performance Liquid Chromatography (UHPLC) Nexera X2 coupled with Triple Quadrupole Mass Spectrometer LCMS-8040.
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Determination of Amantadine Residues in Chicken by Ultra-High-Performance Liquid Chromatography and Triple Quadrupole Mass Spectrometry
This application note describes a method for the determination of amantadine in chicken using Shimadzu Triple Quadrupole Mass Spectrometer LCMS-8050. After extraction and cleanup, the commercially bought chicken sample was separated by Ultra-High-Performance Liquid Chromatograph Nexera X2 and then quantified by LCMS-8050.
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Determination of Amantadine Residues in Chicken by LCMS-8040
A method for the determination of amantadine in chicken was established using Shimadzu Triple Quadrupole Mass Spectrometer LCMS-8040. After extraction and cleanup, the commercially bought chicken sample was separated by Ultra -High-Performance Liquid Chromatograph Nexera X2 and then quantified by Triple Quadrupole Mass Spectrometer LCMS-8040.
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Determination of Twenty-four β-Receptor Agonists in Chicken by Ultra-High-Performance Liquid Chromatography coupled with Triple Quadrupole Mass Spectrometry
Twenty-four β-receptor agonists were determined in chicken sample using Shimadzu Ultra-High-Performance Liquid Chromatograph Nexera X2 coupled with Triple Quadrupole Mass Spectrometer LCMS-8050.
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Determination of Olaquindox Metabolites in Chicken by Ultra-High-Performance Liquid Chromatography coupled with Triple Quadrupole Mass Spectrometry
A method for the determination of olaquindox metabolites (3-methyl-quinoxaline-2-carboxylic acid, MQCA) in chicken using Shimadzu Nexera X2 and Triple Quadrupole Mass Spectrometer LCMS-8040 was developed. After extraction and cleanup, the commercially bought chicken sample was separated by Ultra-High-Performance Liquid Chromatograph and then quantified by Triple Quadrupole Mass Spectrometer LCMS-8040.
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Determination of Sulfonamide Residues in Pork Using LCMS-8045
This application note demonstrates a method for determination of sulfonamide residues in pork using Shimadzu Ultra-High-Performance Liquid Chromatograph (UHPLC) Nexera X2 together with the Triple Quadrupole Mass Spectrometer LCMS-8045.
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Determination of Quinolone Antibiotic Residues in Chicken by Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry
A method was developed for the determination of 12 quinolone antibiotics in chicken using Shimadzu Ultra-High-Performance Liquid Chromatography (UHPLC) Nexera X2 coupled with Triple Quadrupole Mass Spectrometer LCMS-8045.
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LCMS-8040 Application - Multi-Residual Quantitative Analytical Method for Antibiotics in Sea Food by LC/MS/MS
LC/MS/MS method has been developed for quantitation of multi-residual antibiotics from sea food sample using LCMS-8040, a triple quadrupole mass spectrometer from Shimadzu Corporation, Japan. Simultaneous analysis of multi-residual antibiotics often exhibit peak shape distortion owing to their different chemical nature. To overcome this, autosampler pre-treatment feature was used.
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LCMS-8040 Application - Rapid development of quantitative method for determination of plant growth regulators and streptomycin in fruits using LC/MS/MS (India)
Plant growth regulators (PGRs) and antibiotics are commonly used by farmers for enhancing growth of crops and to protect against infections respectively. However, extensive usages of these compounds is known to have adverse effect on human health. Each standard has different linearity range. It is challenging to quickly develop multi-analyte quantitation method for determination of varied compounds simultaneously. One interesting approach can be Method Scouting, which allows user to conduct all method development exercises automatically. This results in swift development of an optimum method which will suit all analytes. Highly sensitive quantitative method was developed quickly using Nexera Method Scouting system for determination of eight PGRs and streptomycin (shown in Figure 1) on LCMS-8040 (shown in Figure 2), a triple quadrupole mass spectrometer from Shimadzu Corporation, Japan.
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HPLC Application - Determination of Polyether Antibiotics in Animal Feeds (Part 1) Analysis by Post-Column VIS Photometric Derivatization Method
Five kinds of polyether antibiotics, including salinomycin sodium, are specified as feed additives based on the 750th notification of Japan's Ministry of Agriculture, Forestry and Fisheries, for addition to the feeds of domestic cattle and poultry to promote growth through the effectiveness of the nutritional content of these feeds. Analysis of these compounds in feed is conducted using microbiological quantitation methods and HPLC methods, as listed in the “Feed Analysis Standard”. HPLC allows more rapid analysis compared with microbiological quantitation methods, with postcolumn derivatization and VIS detection specified for four of the compounds and fluorometric detection specified for one of the compounds. Here we introduce an example of analysis of salinomycin sodium (SL), monensin sodium (MN), narasin (NR) and senduramicin sodium (SD) using the post-column derivatization VIS method, as specified.
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LCMS-2020 Application - Quantitative Analysis of Cholesterol using LCMS-2020 (SSI)
This work demonstrates a rapid method for the detection and quantification of Cholesterol down to a LLOQ of 0.01ppm using a Shimadzu LCMS-2020.
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LCMS-8060 Application- Enhanced Sensitivity for Assay of Sulfonamide Drugs and Trimethoprim in Honey by LCMS Triple Quadrupole Mass Spectrometry with QuEChERS Extraction (SSI)
Twenty one sulfonamides along with trimethoprim were analyzed by LC-MS/MS using a Nexera HPLC system coupled to an LCMS-8060 triple quadrupole mass spectrometer. Diverse clean up sorbents for extraction and purification were tested using a QueChERS method.
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Improving Data Reliability and Laboratory Efficiency Testing of Fluoroquinolones in Fatty and Non-Fatty Fish Samples with One Method using Automated Solid Phase Extraction and New Consumable Technologies
The optimized method parameters showed both high fat and low fat fish samples were effectively recovered following LLE and SPE extraction steps. The larger pore and particle size of the Strata-XL-C SPE cartridges in combination with the SmartPrep Extractor System efficiently automated the combined LLE sample extracts to achieve consistent %RSD values across both high and low-fat fish matrices. Using new column technology with the uHPLC system improved peak resolution for increased sensitivity at the specified 20-ppb FDA monitored level. Elution of all four fluoroquinolone peaks occurred in 7 minutes compared to the 20 minutes using the original column technology .The optimized method passed all FDA criteria for % recovery, % RSD and linearity within less chromatographic time and using current automation and consumable technology. Data presented concludes that while the four fluoroquinolones are structurally similar and exhibit zwitterionic properties, using current consumable chemistries improved overall method efficiency and effectiveness. Improving on work flow by implementing automation for the SPE process with the SmartPrep Extractor enabled objective sample handling across all samples by reducing scientist bias. This improved the reliability of results and the ability to transfer the method to multiple sites for similar results.
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LCMS-8050 Application - Analysis of Residual Chloramphenicol in Shrimp by LC/MS/MS with QuEChERS Sample Pre-treatmen (Asia Pacific)
Chloramphenicol (CAP) is a broad spectrum antibiotic that is widely used in the growth of animals and marine culture. However, CAP can cause aplastic anaemia and is a possible carcinogen in humans. Its use in livestock farming including fish and shrimps has been banned in European Union and many other countries. However, illegal use of CAP is still present due to its low cost and ready availability. As there is not a safe maximum residue level (MRL) determined in food for CAP, a highly selective and sensitive analytical method to detect CAP residues in complex matrices such as food samples is required. Here, we present a LC/MS/MS method for accurate and reliable quantitation of CAP in shrimp samples on LCMS-8050 with a heated ESI interface. The shrimp samples were pre-treated with a modified QuEChERs procedure. The method performance was evaluated with spiked samples and exhibits good accuracy, reproducibility, linearity and specificity over the concentration range from 0.005-20 ng/mL.
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HPLC 3rd Party Application - Improving Data Reliability and Laboratory Efficiency Testing of Fluoroquinolones in Fatty and Non-Fatty Fish Samples with One Method using Automated Solid Phase Extraction and New Consumable Technologies (Horizon)
The optimized method parameters showed both high fat and low fat fish samples were effectively recovered following LLE and SPE extraction steps. The larger pore and particle size of the Strata-XL-C SPE cartridges in combination with the SmartPrep Extractor System efficiently automated the combined LLE sample extracts to achieve consistent %RSD values across both high and low-fat fish matrices. Using new column technology with the uHPLC system improved peak resolution for increased sensitivity at the specified 20-ppb FDA monitored level. Elution of all four fluoroquinolone peaks occurred in 7 minutes compared to the 20 minutes using the original column technology.
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Nexera-I LC2040C 3rd Party Application – Improving the Consistency and Accuracy of Tetracyclines Regulatory Laboratory Testing of Contaminated Beef, Chicken, and Cow’s Milk with Simple Automation (Horizon)
Tetracyclines are one of the most commonly used antibiotic agents in the United States. A majority of the usage is for agricultural purposes, where it is estimated 80% of tetracyclines administered is for disease prevention in healthy animals. They are also used in farming to keep animals raised in close proximity healthy and gaining weight. In 2012, the FDA indicated, tetracycline accounted for 41 percent of the 31 million pounds of antibiotics sold for farm animals in the United States. While the use of tetracyclines is very beneficial for the production of agriculture products, there are concern about long term adverse health effects for humans. Evidence has shown repeated exposure to low levels of tetracyclines through food sources has lead to antibiotic resistant pathogens. Antibiotic resistance to one drug may transfer to another, especially those within the same drug class. Also, repeated exposure to antibiotics could cause the development of allergies to these compounds, especially in children.
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Simultaneous Analysis of 36 Veterinary Drugs using Triple Quadrupole LC/MS/MS
This report illustrates the simultaneous analysis of 36 veterinary drugs measured in 15 minutes using the Shimadzu LCMS-8050 Ultra Fast Triple Quadrupole Mass Spectrometer, featuring ultrafast polarity switching. The polarity switching speed of the LCMS-8050 is just 5 milliseconds.
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Quantitative Analysis of Veterinary Drugs Using the Shimadzu LCMS-8050 Triple Quadrupole Mass Spectrometer
Foods in which chemical residues, like pesticides, feed additives, and veterinary drugs found in excess of maximum residue levels have been banned from sale in many countries around the world. Compounds that are subject to residue standards vary widely and the list is expected to grow. Because of this, there is a need for a highly sensitive and rapid analytical technique to analyze as many of these compounds as possible in a single run. This Application News introduces an example of the high-sensitivity analysis of 89 veterinary drugs in a crude extract of livestock and fishery products.
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LCMS-8050 Application - Low Level Quantitation of Steroids in Milk using LC/MS/MS (India)
A highly specific LC/MS/MS method has been developed for trace level quantitation of steroids in milk using LCMS-8050, a triple quadrupole mass spectrometer from Shimadzu Corporation, Japan. Ultra high sensitivity of LCMS-8050 due to heated ESI source, enabled development of ppt level quantitation for testosterone, progesterone and estradiol.
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LCMS-8040 Application - Determination of 68 Veterinary Drugs in Marine Products by Ultra High Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (China)
Pesticides are widely used in crop planting and growth process. However, residual pesticides in food could enter human body and harm to human health. Therefore, the maximally allowed residual pesticides in food have strictly regulated in the world. In recent years, the China government continues to strengthen supervision and is developing quicker and highly sensitive analytical method. Usually, the qualitative LC/MS/MS is based on the ratio of intensities between targetions and reference ions. But in some special occasions, especially for determination of low concentration samples, qualitative reliability is not high enough, which may cause false positive/negative results. So use of MS/MS library for screening and qualitative confirmation of the unknown pesticides is very meaningful. This paper describes UHPLC/MS/MS, a rapid and accurate screening method for determination of 200 residual pesticides in agricultural products.
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Particle Size Distribution Measurement of Chocolate
This article describes the use of a SALD-2300 laser diffraction particle size analyzer to measure the size distribution of particles in chocolate. Chocolate is a mixture of cocoa mass, which consists of ground cocoa beans mixed with milk, sugar, cocoa butter, and other ingredients. This can be considered as a mixture of various particles in fat. Consequently, the particle size distribution presumably varies depending on the dispersion conditions. In this example, isopropanol at about 45 °C was used as the dispersant.
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High Speed Analysis of Mycotoxins
Mycotoxins are low-molecular-weight natural products produced by fungi and are capable of causing disease and death. Mycotoxins are typically found in grain products and their occurrence is increased under certain heat and humidity conditions. Regulatory levels have been set for mycotoxin levels, and are dependent on the end use of the food products. Mycotoxin levels can vary widely within the same crop, creating a potential need for increased testing to provide more accurate mycotoxin levels. A high-speed UHPLC method was developed that could provide separation of eight common mycotoxins in less than two minutes to allow for increased testing. Details of the method will be presented.
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