UV-Vis Frequently Asked Questions - Reflection Spectroscopy

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What are the different types of reflection?

Different types of reflection diagram

Reflectance is measured by shining light on a sample and measuring the light reflected from the sample. Reflected light consists of specular reflected light and diffuse reflected light, which when combined is referred to as total reflected light (specular reflected light plus diffuse reflected light). The figure here shows a diagram of these types of reflected light. The light shone onto the sample is called the incident light and the angle formed between the incident light and sample is called the angle of incidence. The incident angle is represented with the symbol θ.

Specular reflected light is the light reflected from shiny mirror-like surfaces at the same angle as the incident angle. Diffuse reflected light is the diffuse light reflected in all directions from rough surfaces, such as paper and powder surfaces. Total reflected light is the total combination of specular and diffuse reflected light. Total reflected light is often measured when measuring samples that have both rough and shiny characteristics, such as plastic and painted samples. The methods used to measure specular, diffuse, and total reflected light are referred to as specular, diffuse, and total reflected light measurement methods, respectively.

What are the different modes of measuring reflection?

 

Different modes of measuring reflection

Reflectance measurements measure either the relative or absolute reflected light, with measurement values expressed in terms of reflectance.

Relative reflectance measurements calculate the proportional amount of reflected light measured from a sample surface, relative to the amount of reflected light measured from a reference plate, such as barium sulfate or a mirror. The relative reflectance is calculated based on assuming the reference plate has a reflectance of 100 %. Therefore, it is very important to manage reference plates properly because different reflectance values can be obtained if reference plates are substituted or if they become contaminated or change characteristics.

In contrast, absolute reflectance measurements calculate the proportional amount of reflected light relative to the amount of light measured directly from a light source, not using a reference plate such as barium sulfate or a mirror. Reflectance measurement values are based on assuming a 100 % reflectance for air. Absolute reflectance measurements allow for the quantification of the true reflectance of samples, which is referred to as absolute reflectance.

For Research Use Only. Not for use in diagnostic procedures.

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