Exploring Factors that Influence Charge Variant Analysis of mAbs with Shim-pack Bio IEX Columns using Salt Gradient Elution

In this study, we describe a charge variant analysis of Trastuzumab using salt gradient with Shimadzu strong cation-exchange column, Shim-pack Bio IEX SP-NP. The separation efficiency under different gradient slopes, gradient times and flow rates are discussed.

Content Type:
Application News
Document Number:
AD-0227
Product Type:
High Performance Liquid Chromatography (HPLC & UHPLC)
Keywords:
therapeutic drugs, monoclonal antibodies, mabs, glycostation, oxidation, c-terminal, lysine, cleavage, iex, Pharma & Biopharma, Monoclonal Antibodies and Biosimilars, nexera bio uhplc
Language:
English
File Name:
shimadzu-ad-0227.pdf
File Size:
430kb

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For Research Use Only. Not for use in diagnostic procedures.

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